Blood Practicum III and IV

Blood Practicum III and IV
(Hemolisa and Krenasi, Erythrocyte Osmotic Pressure, Density of Blood, Blood, Blood Pressure)
 

The purpose of this lab is to to find a large blood pressure, blood hemoglobin concentration, hematocrit value of blood, to determine the number of red blood cells and white blood cells per mm3 of blood volume, and the factors that influence it. Usefulness is to be aware of and comparative assessment of blood pressure in various activities, knowing the blood hemoglobin concentration, hematocrit value of blood knows and can know the number of red blood cells (erythrocytes) and white blood cells (leukocytes) in blood per mm3 of blood volume. 

The tools used are landset pen, room count, cover glass, microscope, scale paper hemoglobin, Sahli tubes, micro-centrifuge, haemoglobinometer Sahli, mikrokapiler, micro-hematocrit, pipette suction, the cover glass. Materials used are cotton, 0.1 N HCl, blood samples, 70% alcohol, hayem solution, a solution of turk, aquades, lancet pen, the capillary tube. Practical method of taking blood samples and compare to the scale specified levels of hemoglobin and hemoglobin. Place the tip of the capillary tube at the fingertips. Let go with his own blood until 4 / 5 sections of pipe length, then insert it into the micro centrifuge and centrifuge for 2 minutes. Read the results. Sucking blood using red blood cell pipette to 0.5. Then suck up to number 101 hayem solution and then put it on the room count at the edge of the room count. Check with a microscope at a big enlargement. The calculation is performed on red blood cells contained in the five fruit box marked R. The number of red blood cells obtained in five such boxes 10,000 for dilution multiplied 200 times, and multiplied by 5000, to 100 times dilution. Sucking blood until a solution of turk 0.5 and then suck until angka11 with caution. The next procedure similar to the calculation of red blood cells.

Practicum Blood Part I (Physiology courses livestock)

Practicum Blood Part I (preparations Natip Blood, Time Frozen Blood, Bleeding time and erythrocyte sedimentation rate).

This practicum aimed to see how the shape of blood cells, blood cells function, length of time frozen blood, coagulation time, bleeding time and the time of erythrocyte sedimentation rate. The usefulness of this lab is for students to know the shape of blood cells, blood cells function, length of time frozen blood, coagulation time, bleeding time and time erythrocyte sedimentation rate.

The tools used are glass objects, the cover glass, microscope, paraffin-coated petri dish, stopwatch, along westergrin tube rack, test tube and rack, lancet pen, capillary tube, laktodencimeter. While the materials used are chicken blood, chicken, cows, humans (male and female), cotton, filter paper, anticoagulant EDTA, 0.9% NaCl solution and 70% alcohol. Practical method that is dripping 1-2 drops of 0.9% NaCl solution on the object glass and blood drawn from fingertip aseptically. Mixing the two kinds of solution and then observed with a microscope. Removing the blood from a fingertip and blood was dripping 1-2 drops onto a petri dish. Prick blood was slowly picking up slowly with the use of a pin. Noted the time. Entering the blood coming out of the fingertips into the capillary tube until 4 / 5 length of pipe by means stick one end and then broke the 1 cm piece of pipe threads to form fibrin, while noting the time koagulasinya, bleeding from the fingertips using the lancet pen. suck every drop of blood with filter paper until no more blood stains on filter paper. Noted the time. Provided blood samples which have been anticoagulant Entering mixed blood sample into the Westergren tube and let it stand upright. Noting how many mm decrease in erythrocytes after 30, 60 and 90 minutes.